Steele Lab Hydra Inventory

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These are the strains we currently have in the lab. Most of them are kept as small cultures. The only strains for which we maintain large cultures are 105, AEP, and Zurich.

Hydra magnipapillata strain 105 - This is the strain whose genome was sequenced for the Hydra Genome Project. This strain was also used to construct most of the cDNA libraries used for the Hydra EST Project. This strain originated from a single polyp collected by Dr. Tsutomu Sugiyama in September, 1973 in a swamp adjacent to the National Institute of Genetics in Mishima, Japan (Dr. Toshitaka Fujisawa, unpublished information). This strain is widely used for research and is maintained in labs in Japan, Europe, and the United States. This strain responds well to diacylglycerol treatment by forming ectopic heads. This strain forms ectopic tentacles when treated with alsterpaullone.

Hydra vulgaris strain AEP - The AEP strain of Hydra vulgaris is used to generate embryos for production of transgenic Hydra. AEP produces both sperm and eggs, and does so pretty much continuously. Most of the time a given sexual polyp contains only testes or eggs, although occasionally a polyp is seen which contain both testes and an egg. The details of how this strain was generated are not completely clear. Everyone agrees that the founders for this strain were a male strain called CA7, collected from Boulder Creek, south of Susanville, California by Lynne Littlefield and Carolyn Teragawa and a female strain, called PA1, collected from a pond on the Haverford College campus by Carolyn Teragawa. It appears that Boulder Creek drains into the north side of Antelope Lake. Haverford College is in Haverford, Pennsylvania, which is near Philadelphia. There is a single large pond on the campus called the Duck Pond. It seems likely that this is the pond from which PA1 was collected. Both CA7 and PA1 were collected sometime between the late 80s and the early 90s. Lynne and Carolyn were both postdocs in Hans Bode's lab at UC Irvine at the time the CA7 and PA1 strains were brought into the laboratory.

All those involved are in agreement that the initial step in generating the AEP strain was a cross between CA7 and PA1. This cross gave rise to multiple lines designated with letters A, B, C, D, E, etc. Vicki Martin et al. (1997) mention a male E2 line and a female A5 line, which must be from this cross. Pat Bode recalls that subsequently crosses among these letter-designated lines were carried out. It is presumed that an AE line was generated by crossing of an A line and an E line. Which A line and which E line were used in this cross is not currently known. The logical final step in the generation of AEP would have been a cross between the presumed AE line and a P line. So AE could have been backcrossed to PA1 to generate AEP.

In an e-mail sent in 2005, Vicki Martin said she thought that a line called PA2 was generated from a cross between CA7 and PA1 and that a cross between a PA2 male and a PA2 female gave rise to AEP. This seems unlikely for two reasons. First, Pat Bode is certain about the production of the A, B, C, D, E, etc. lines from a CA7/PA1 cross and she is certain that crosses among those lines were then carried out. Second, Pat’s recollection makes sense in terms of the nomenclature for AEP. It seems unlikely that AEP would be chosen as the name if the cross was done as Vicki thinks, and Vicki says she doesn’t remember why the line was called AEP. Technau et al. (2003) report crossing AEP females to PA2 males, indicating that a male line called PA2 did, apparently, exist at one point.

Unless documentation is found of exactly how the crosses beyond the initial CA7/PA1 cross were carried out, it won’t be possible to determine the complete pedigree of AEP with certainty.

Hydra vulgaris strain Zurich - This strain was obtained from Monika Hassel's lab when she was at the University of Heidelberg. It was originally collected by Pierre Tardent from a lake in Zurich, Switzerland. It responds to treatment with lithium chloride by forming ectopic feet.

Hydra vulgaris from Newport Beach - collected from a small pool located adjacent to Upper Newport Bay.

Hydra oligactis from Daniel Martinez -

Hydra utahensis from Daniel Martinez -

Hydra hymenae from Daniel Martinez -

Hydra vulgaris from Australia - This Hydra strain shows the peculiar property of undergoing transverse fission of the polyp at the top of the peduncle just beneath where a bud has emerged.

Hydra recently obtained from Dick Campbell

1873c collected 27 Aug 08, Canada: Alberta:NW of Edmonton: along Twp Rd 564, 0.7 km E of Rd 263. This is close to the type locality for Hydra carnea and fits the description; similar to 950f. H vulgaris is a better name. This is a very sexy strain, protandric, makes lots of eggs.

1869. H. utahensis. collected 27 Aug 08: Canada: Alberta: St Albert: Sturgeon River, 1000' W Highway #2. This is a small version of H. hymanae, the 2 North American species in the braueri group. It is hermaphroditic, and egg theca flatten somewhat against the substratum.

1891b H. oligactis collected 31 Aug 08: New York: Orange Co.: Monroe: Orange & Rockland Lake, above dam on Museum Village Road.

1907h H. hymanae collected 02 Sept 08: Illinois: Cook Co.: Chicago: Jackson Park, near southern bridge.

1921m H. vulgaris (small) collected: 9 Sept 08: California: San Diego Co.: Santee Lakes, west side just north of the Mast Road overpass. Mainly female but occasional males appear too.

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