Annotation and analysis of the Hydra genome sequence
From Steele Lab
History of the Hydra Genome Project
Sequencing of the Hydra genome was recommended by the Comparative Genome Evolution Working Group at the National Human Genome Research Institute (NHGRI) in a proposal issued on July 29, 2004. Approval for sequencing of 18 organisms, including Hydra, was announced in an NHGRI press release on August 4, 2004. Hydra magnipapillata strain 105 was chosen as the strain to be sequenced because of its widespread use for Hydra research and the fact that it was used for construction of most of the cDNA libraries for the Hydra EST Project. In addition this strain was the species used for the Hydra Peptide Project.
Sequencing for the Project was carried out at the J. Craig Venter Institute. The assembled sequence was submitted to GenBank on July 14, 2008. The Hydra Genome Project was preceded by two Hydra EST projects. One of the projects was carried out at the National Institute of Genetics in Mishima, Japan. The second project was funded by a grant from the National Science Foundation awarded to Hans Bode and Rob Steele and was carried out by the Genome Sequencing Center at Washington University.
Construction of Libraries and Sequencing
DNA for sequencing was obtained from a sample of Hydra magnipapillata strain 105 provided to the Venter Institute by Hans Bode's lab at UC Irvine.
Hydra Genome Assemblies
Two assemblies were done with the sequences. The one done by the Venter Institute has been deposited in GenBank under accession number ABRM00000000. A second assembly was done by Dan Rokhsar's lab at UC Berkeley. This assembly has been deposited in GenBank under accession number ACZU00000000. The Berkeley assembly is also accessible through the Hydra genome browser set up and maintained by the Rokhsar lab.
The Hydra Genome Browser
A GBrowse type browser for the Hydra genome has been set up and is maintained by Dan Rokhsar's lab at UC Berkeley. The browser is built on an assembly of the Hydra genome carried out by Jarrod Chapman. Click here to connect to the Hydra Genome Browser.